FOCμS LAB
Fabrication of Organoid and Complex μ (micro/nano)-scale biomimetic Surfaces Laboratory
Kidambi Lab
PEER-REVIEWED MANUSCRIPTS (* corresponding author)
38)“Alcohol metabolism potentiates HIV-induced hepatotoxicity: Contribution to end-stage liver disease”, Murali Ganesan, Moses New-Aaron, Raghubendra Dagur, Edward Makarov, Weimin Wang, Kusum Kharbanda, Srivatsan Kidambi, Larisa Poluektova, Natalia Osna, Biomolecules, 2019
39)“Ethanol impairs NRF2/antioxidant and growth signaling in the intact placenta in vivo and in human trophoblasts”, Sambanthan Shanmugam, Dhyanesh Patel, John Wolpert, Caezaan Keshvani, Xiaobo Liu, Susan Bergeson, Srivatsan Kidambi, Lenin Mahimainathan, George Henderson, Madhusudhanan Narasimhan, Biomolecules, 9, 669, 2019
36) “Depletion of mitochondrial protease OMA1 alters proliferative properties and promotes metastatic growth of breast
cancer cells”, Amita Daverey, Roman Levytskyy, Samantha Swenson, Kimberly Stanke, Stephen Hayward, Madhu
Narasimhan, Oleh Khalimonchuk*, Srivatsan Kidambi*, Nature Scientific Reports, 9, 14746, 2019
35)“A totally recombinant fibrin matrix for mesenchymal stem cell culture and delivery”, Ou Wang, Ayman Ismail, Frank
Fabian, Haishuang Lin, Qiang Li, Christian Elowsky, Mark Carlson, Wilson Burgess, William Velander, Srivatsan Kidambi,
Yuguo Lei, Journal of Biomedical Materials Research-Part A, 2018
34)“Traumatic Injury in Astrocytes: An In Vitro Study”, Yi Hua, Christina Wilson, Shengmao Lin, Diganta Dutta, Srivatsan
Kidambi, Linxia Gu, Journal of Mechanics in Medicine and Biology , 18, 4, 2018
33)“CD44 Targeted Lipid Nanoparticles for MicroRNA Therapy”, Stephen Hayward, Srivatsan Kidambi*, Methods in Molecular Biology-Targeted Drug Delivery, 1831, 95-109, 2018.
32) “Matrix Stiffness Regulate Apoptotic Cell Death in HIV-HCV co-infected Hepatocytes: Importance for Liver Fibrosis
Progression”, Murali Ganesan, Raghu Dabur, Eric Makarov, Larisa Poluektiva, Srivatsan Kidambi, Natalia Osna,
Biochemical and Biophysical Research Communications, 500, 3, 717-722, 2018
31) “In vitro studies of alcohol-induced liver injury in virally-infected human hepatocytes: Advantages and limitations", Natalia
Osna, Srivatsan Kidambi, International J Hepatobiliary Pancreatic Diseases, 2017.
30) “SECs (Sinusoidal Endothelial Cells), Liver Microenvironment, and Fibrosis”, Vaishaali Natarajan, Edward Harris,
Srivatsan Kidambi*, BioMed Research International, 2017
29) “Three-dimensional co-cultures of human coronary artery endothelial and smooth muscle cells: A novel in-vitro
experimental model of atherosclerosis” Martin Liu, Angelos Karagiannis, Matthew Sis, Nikolaos Tsigkas, Erik Rask,
Srivatsan Kidambi, Yiannis Chatzizisis, Atherosclerosis, 2017. doi: 10.1016/j.atherosclerosis.2017.06.257.
28) “Role of Matrix Rigidity on Hepatocytes and LSECs in Alcohol Induced Hepatic Fibrogenesis” Vaishaali Natarajan,
Edward Harris, Carol Casey, Srivatsan Kidambi*, Alcohol and Alcoholism, doi: 10.1093/alcalc/agx075.60, 2017.
27) “Scalable Production of Glioblastoma Cancer Stem Cells in 3 Dimension Thermoreversible Hydrogels", Qiang Li,
Haishuang Lin, Ou Wang, Xuefeng Qiu, Srivatsan Kidambi, Loic P Deleyrolle, Brent Reynolds, and Yuguo Lei, Nature
Scientific Reports, 6, 31915, 2017
26) “Drug-Loaded Particles: "Trojan Horses" in the Therapy of Atherosclerosis”, Nikolaos Tsigkas, Srivatsan Kidambi, Ahmed
Tawakol, Yiannis S. Chatzizisis, Atherosclerosis, 2016, doi: 10.1016/j.atherosclerosis.2016.06.050
25) “Astrogliosis in a Dish: Substrate Stiffness Induces Astrogliosis in Primary Rat Astrocytes” Christina Wilson, Stephen
Hayward, Srivatsan Kidambi*, RSC Advances, 6, 34447-34457, 2016, doi: 10.1039/C5RA25916A
24) “Hyaluronic Acid-Conjugated Liposome Nanoparticles for Targeted Delivery to CD44 Overexpressing Glioblastoma
Cells” Stephen Hayward, Christina Wilson, Srivatsan Kidambi*, Oncotarget, 2016, doi: 10.18632/oncotarget.8926
23) “Targeted Delivery of MicroRNA-125a-5p by Engineered Lipid Nanoparticles for the Treatment of HER2 positive
Metastatic Breast Cancer”, Stephen Hayward, David Francis, Parviz Kholmatov, Srivatsan Kidambi*, Journal of
Biomedical Nanotechnology, 12(3), 554-68, 2016 doi: 10.1166/jbn.2016.2194
22) “Substrate Elasticity Regulates Primary Hepatocytes Functions”, Vaishaali Natarajan, Eric Berglund, Dorothy Chen,
Srivatsan Kidambi*, RSC Advances, 5, 80956-80966, 2015, doi: 10.1039/C5RA15208A
21) “Ionic Driven Embedment of Hyaluronic Acid Coated Liposomes in Polyelectrolyte Multilayer Films for Local Therapeutic
Delivery”, Stephen Hayward, David Francis, Matthew Sis, Srivatsan Kidambi*, Nature Scientific Reports, 5, 14683, 2015,
doi: 10.1038/srep14683
20) “In vitro Co-cultures of Breast Cancer Cells and Stromal Cells to Emulate Tumor Progression and miRNA Profiles of
Clinical Conditions”, Amita Daverey, Karleen Brown, Srivatsan Kidambi*, Langmuir, 31(36), 9991-10001, 2015 doi:
10.1021/acs.langmuir.5b02227
19) “Mitochondrial Dysfunction and Loss of Glutamate Uptake in Primary Astrocytes Exposed to Titanium Dioxide
Nanoparticles”, Christina Wilson, Vaishaali Natarajan, Stephen Hayward, Oleh Khalimonchuk, Srivatsan Kidambi*,
Nanoscale, 7(44), 18477-88, 2015 doi: 10.1039/c5nr03646a
18) “Physical Intimacy of Breast Cancer Cells with Mesenchymal Stem Cells Elicits Trastuzumab Resistance through Src
Activation” Amita Daverey, Allison Drain, Srivatsan Kidambi*, Scientific Reports, 5, 13744, 2015, doi: 10.1038/
srep13744
17) “Mitochondrial Dysregulation and Loss of Hepatic Functions due to Chronic Exposure to Titanium dioxide Nanoparticles to
Primary Hepatocytes”, Vaishaali Natarajan, Christina Wilson, Stephen Hayward, Srivatsan Kidambi*, PLOS One, 10(8),
e0134541, 2015 doi: 10.1371/journal.pone.0134541
16) “Dye-doped silica encapsulated magnetic nano-assemblies for dual optical/MRI imaging and hyperthermia", Sunil Kumar,
Dhirendra Bahadur, Amita Daverey, Vahid Khalilzad-Sharghi, Niroj Sahu, Srivatsan Kidambi, Shadi Othman, RSC
Advances, 5, 53180-53188, 2015 doi: 10.1039/C5RA07632C
15) “FMR1 Deficiency Alters Self-Renewal, Proliferation, and Pluripotency of Mouse Embryonic Cells”, Shyam Sundhar Bale;
Nima Saeidi; Srivatsan Kidambi; Martin Yarmush; Monica Casali, NanoLife, 5(2), 15500, 2015. doi: 10.1142/
S1793984415500038
14) “Topography Mediated Regulation of HER-2 Expression in Breast Cancer Cells” Amita Daverey, Austin Mytty, Srivatsan
Kidambi *, NanoLife, 2(3), 12410, 2012 doi: 10.1142/S1793984412410097
13) “Propofol induces MAPK/ERK cascade dependant expression of c-Fos and Egr-1 in rat hippocampal slices” Srivatsan
Kidambi*, Joel Yarmush, Yevgeny Berdichevsky, Sangeetha Kamath, Wayne Fong, Joseph SchianodiCola, BMC Research
Notes 17(3), 201-206, 2010
12) “Oxygen-Mediated Enhancement of Metabolism, Functional Polarization, Gene Expression an Drug Clearance in
Cocultures of Primary Hepatocytes” Srivatsan Kidambi, Eric Novik, Ruby Yarmush, Piyun Chao, Martin Yarmush, Yaakov
Nahmias, PNAS, 106(37), 15714-9, 2009.
11) “Propofol induces a Dose-Dependent Alteration in the Expression of c-Fos and Egr-1 in Neuronal Cells” Srivatsan
Kidambi*, Joel Yarmush, Wayne Fong, Sangeetha Kamath, Joseph SchianodiCola, Yaakov Nahmias, NeuroReport, 20(7),
657-662, 2009.
10) “Tunable Resistive m-dPEG Acid Patterns on Polyelectrolyte Multilayers at Physiological Conditions: Template for
Directed Deposition of Biomacromolecules” Srivatsan Kidambi, Christina Chan*, Ilsoon Lee, Langmuir, 24(1),224-30,
2008.
9) “Patterned Co-culture of Neurons and Astrocytes on Polyelectrolyte Multilayer Films for Studying Astrocyte Mediated
Oxidative Stress in Neurons” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. Advanced Functional Materials, 18,
294-301, 2008.
8) “Selective Adhesion of Primary Hepatocytes on Polyelectrolyte Multilayers: Template for Patterned Cell Co-
culture” Srivatsan Kidambi, Lufang Sheng, Mehmet Toner, Martin Yarmush, Ilsoon Lee, Christina Chan. Macromolecular
Biosciences, 7(3), 344-53, 2007.
7) “Cell Adhesion on Polyelectrolyte Multilayer coated PDMS Surfaces with Varying Topographies” Srivatsan Kidambi,
Natasha Udpa, Stacey Schroeder, Ilsoon Lee, Christina Chan. Tissue Engineering, 13 (8), 2105-2117, 2007.
6) “Salt tunable m-dPEG Acid Patterns on Polyelectrolyte Multilayer - Templates for Directed Deposition of
Macromolecules” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. Polymer Preprints, 47(1), 4
5) “Polyelectrolyte Multilayer as Bioactive Surface for Cell Adhesion” Srivatsan Kidambi, Ilsoon Lee, Christina Chan.
Polymer Preprints 46(2), 1271-1272, 2005
4) “Selective Primary Hepatocyte Adhesion on Polyelectrolyte Multilayer: Template for Patterned Cell Co-culture” Srivatsan
Kidambi, Ilsoon Lee, Christina Chan. AICHE Preprint, 2004.
3) “Engineering Polyelectrolyte Multilayer (PEM) Surfaces to Create Cell Resistant and Adhesive Surfaces” Srivatsan Kidambi,
Ilsoon Lee, Christina Chan. Polymer Preprints 45(1), 88-89, 2004.
2) “Controlling Primary Hepatocyte Adhesion and Spreading on Protein Free Polyelectrolyte Multilayer Films” Srivatsan
Kidambi, Ilsoon Lee, Christina Chan. Journal of the American Chemical Society, 126 (50), 16286 -16287, 2004.
1) “Selective Depositions on Polyelectrolyte Multilayers: Self-Assembled Monolayers of m-dPEG Acid as Molecular
Templates” Srivatsan Kidambi, Christina Chan, Ilsoon Lee., Journal of the American Chemical Society, 126,
4697-4703, 2004.
MANUSCRIPTS UNDER PROCESS (* corresponding author)
1) “Stiffness Induces NAFLD-Like Metabolic Dysfunction in Primary Hepatocytes”, Michael Moeller, Senthil Thulasingam, Madhu Narasimhan, Srivatsan Kidambi*, under review
2)“Effects of Stiffness on Hepatocyte-Non parenchymal Cell Communication”, Youra Moeun, Vaishaali Natarajan, Michael Moeller, Srivatsan Kidambi*, under review
3)“Matrix Stiffness Regulate Liver Sinusoidal Endothelial Cells (LSECs) Function: Importance for Liver Fibrosis Progression”, Vaishaali Natarajan, Carol Casey, Edward Harris, Srivatsan Kidambi*, under review
4)“Direct Contact of GBM cells with Astrocytes drives metabolic reprogramming of Glioma Cells” Kimberly Stanke, Christina WIlson, Oleh Khalimonchuk, and Srivatsan Kidambi*, under review.
5)“Substrate Stiffness Regulates CD44 Receptor Mediated Endocytosis of Liposomal Nanoparticles”, Stephen Hayward, Srivatsan Kidambi*, under review
6)“High Expression of Glycolytic Genes in GBM Correlates with Lower Survival Rates” Kimberly Stanke, Carrick Wilson, and Srivatsan Kidambi*, under review.
7)“Tunable Modulation of Breast Cancer Biology due to Dynamic Well-Defined Mechanical Microenvironment”, Amita Daverey, Ravi Saraf, Srivatsan Kidambi, under review
8)“METH elicits dose- and time-dependent autophagic cytotoxicity in primary astrocytes” Christina Wilson, Kimberly Stanke, Rodrigo Franco Cruz, Rick Bevins, Oleh Khalimonchuk, and Srivatsan Kidambi*, under review.
9)“Nanoceria elicits shape- and size-dependent toxicity in Primary Hepatocytes”, Vaishaali Natarajan, Barry Cheung, Srivatsan Kidambi*, under review.
PATENTS
1“Mitochondrial Protease OMA1 as a Marker for Breast Cancer” WO2018132662A1
2“Substrate Delivery of Embedded Liposomes” WO2017031060A1
3“Cell Adhesion on Surfaces of Varying Topographies” US 20080241926
4“Salt and pH tunable Polyelectrolyte Multilayers for uses as bioengineering scaffolds” US 20070224175
5“Development of Polyelectrolyte Multilayer Coatings for Primary Hepatocyte Cell Culture System” (MSU Invention Number 06-026).
6“Aptamer Arrays on Polyelectrolyte Multilayer Films” (MSU Invention Number 05-117F).
MEETINGS AND CONFERENCES
Published Abstracts and Proceedings
1“Salt tunable m-dPEG Acid Patterns on Polyelectrolyte Multilayer - Templates for Directed Deposition of Macromolecules” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. Polymer Preprints, 47(1), 41-42, 2006.
2“Polyelectrolyte Multilayer as Bioactive Surface for Cell Adhesion” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. Polymer Preprints 46(2), 1271-1272, 2005.
3Selective Primary Hepatocyte Adhesion on Polyelectrolyte Multilayer: Template for Patterned Cell Co-culture” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. AICHE Preprint, 2004.
4“Engineering Polyelectrolyte Multilayer (PEM) Surfaces to Create Cell Resistant and Adhesive Surfaces” Srivatsan Kidambi, Ilsoon Lee, Christina Chan. Polymer Preprints 45(1), 88-89, 2004.
Invited Talks
1.“Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Department of Materials Science and Engineering, University of Michigan-Ann Arbor, Ann Arbor, MI, Feb, 2016
2.“Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Nebraska Center for the Prevention of Obesity Diseases Seminar Series, University of Nebraska-Lincoln, Lincoln, NE, Nov, 2015
3.“Matrix based liver models to investigate the role of microenvironment on liver fibrosis”, International Symposium on the Cells of the Hepatic Sinusoid, Asilomar, CA, Nov, 2015
4.“Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Fred & Pamela Buffett Cancer Center Grand Rounds, University of Nebraska Medical Center, Omaha, NE, Nov, 2015
5.“Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Big Ten Seminar Exchange, Department of Biomedical Engineering Northwestern University, Chicago, IL, Nov, 2015
6.“Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Regenerative Medicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, Oct, 2015
7.“WHAT DOES THE STROMAL CELLS SAY (TO THE TUMOR CELLS)? One sound that no one knows….”, Regenerative Medicine, University of Nebraska Medical Center, Omaha, NE, June, 2015
8.“Engineering Nanostructured Polymer Films for In Vitro Models of Tissue”, Indian Institute of Technology, Madras, India, September, 2014
9.“Molecular bases of the role of METH-induced inflammation on behavioral abnormalities”, National Institute of Drug Abuse, Bethesda, MD, June, 2014
10. “Engineering Nanostructured Polymer Films for In Vitro Models of Liver”, University of Nebraska-Lincoln Interdisciplinary Retreat, Lincoln, NE, May, 2014
11. “Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, University of Nebraska-Lincoln Department of Animal Science, Lincoln, NE, Oct, 2013
12. “In Vitro Liver Models-Design and Cellular Interaction Studies”, International Symposium on the Cells of the Hepatic Sinusoid, Osaka, Japan, Sep, 2013
13. “Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Regenerative Medicine, University of Nebraska Medical Center, Omaha, NE, Apr, 2013
14. “Engineering Nanostructured Polymer Films for In Vitro Models of Living Tissues”, Center for Nanohybrid Functional Materials, University of Nebraska, Lincoln, NE, Mar, 2013
15. “Propofol induces MAPK/ERK cascade dependant expression of c-Fos and Egr-1 in brain”, Department of Anesthesiology, NY Methodist Hospital, Brooklyn, NY, Aug, 2010.
16. “Engineering Novel Polyelectrolyte Multilayer Films for Tissue Engineering and Drug Delivery Applications”, Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, June, 2009.
17. “Engineering Novel Polyelectrolyte Multilayer Films for Tissue Engineering and Drug Delivery Applications”, Department of Biomedical Engineering, University of Akron, Akron, OH, April, 2009.
18. “Engineering Novel Polyelectrolyte Multilayer Films for Tissue Engineering and Drug Delivery Applications”, Department of Chemical and Biomolecular Engineering, University of Akron, Akron, OH, April, 2009.
19. “Nanotechnology Approaches for Tissue Engineering and Drug Delivery Applications”, Department of Chemical Engineering, Western Michigan University, Kalamazoo, MI, March, 2009.
20. “Engineering Novel Polyelectrolyte Multilayer Films for Tissue Engineering and Drug Delivery Applications”, Department of Chemical and Biomolecular Engineering, Nanyang Technological University, Singapore, March, 2009.
21. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Immune Disease Institute, Harvard Medical School, Boston, MA, November, 2008.
22. “Salt Tunable m-dPEG Acid Patterns on Polyelectrolyte Multilayer - Templates for Directed Deposition of Macromolecules”, Polymer Science Group, Symyx, Santa Clara, CA, November, 2008.
23. “Salt tunable m-dPEG Acid Patterns on Polyelectrolyte Multilayer - Templates for Directed Deposition of Macromolecules”, Hitachi Chemical Research Center, Irvine, CA, September, 2008.
24. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Symyx, Santa Clara, CA, September, 2008.
25. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Eli Lilly, Indianapolis, IN, August, 2008.
26. “Gene Expression Changes in Brain due to Propofol” Department of Anesthesiology, New York Methodist Hospital, Brooklyn, NY, May, 2008.
27. “Aptamer Incorporated Polyelectrolyte Multilayer Films Targeting Influenza Virus’s Hemagglutinin Binding Region” 234th ACS National Meeting, Boston, MA, August, 2007. (ICI Student Award Finalist in Applied Polymer Science)
28. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Centre for Engineering in Medicine, Harvard Medical School, MGH, Boston, MA, October, 2006.
29. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, July, 2006.
30. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, June, 2006.
31. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Amgen, San Francisco, CA, March, 2006.
32. “Engineering Invitro Cellular Microenvironments using Polyelectrolyte Multilayer Films and Microfabrication Techniques to Control Cell Adhesion and for Drug Delivery Applications”, Intel, Portland, OR, November, 2005.
33. “Synthesis and Characterization of Novel Cellulose Ethers from Environmentally Friendly Starting Products”, Merck, West Point, PA, November, 2002.